Overview
- Requires the PCR Barcoding Expansion (EXP-PBC001 or EXP-PBC096) with the Ligation Sequencing Kit V14 (SQK-LSK114)
- This protocol barcodes gDNA or amplicons
- Library preparation time ~150 minutes + PCR
- High yield
- Uses up to 96 barcodes
- Includes PCR steps
- Compatible with R10.4.1 flow cells
For Research Use Only
Introduction to the protocol
Library preparation
Sequencing and data analysis
Troubleshooting