21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
- Added amplicon tailed primer sequences to the introduction
May 2023
- Added flow cell priming mix volumes to the "Priming and loading" steps
- This kit has moved to released phase.
April 2023
- Added flow cell priming mix volumes to the "Priming and loading" steps
March 2023:
- Updated how much DNA is expected and taken forwards from the first end-prep step
March 2023:
- Added instructions for preparing ONT reagents
March 2023:
- Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.