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DNA extraction
Extract DNA from your sample using one of our recommended extraction protocols. For the benchmarking of this method, the Oxford Nanopore team extracted DNA from ~5 million cells using the protocol: Human cell line DNA – QIAGEN Puregene Cell Kit. We also offer multiple mammalian sample extraction protocols, which you can use for other sample types.
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DNA fragmentation
The DNA was sheared using the protocol for Covaris g-TUBE fragmentation, with the following modifications:
- The input was 8 μg of DNA in 150 μl.
- The DNA was fragmented in the g-TUBE at 11,000 rpm in 30 sec pulses. The resulting fragment length should be ~6-7 kb.