- Materials
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- 100 ng genomic DNA
- Consumables
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- g-TUBE™ (Covaris, Cat. # 520079)
- 1.5 ml Eppendorf DNA LoBind tubes
- Equipment
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- Microfuge
- P100 pipette and tips
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Prepare the DNA in nuclease-free water.
- Transfer 100 ng genomic DNA into a 1.5 ml Eppendorf DNA LoBind tube
- Adjust the volume to 50 μl with nuclease-free water
- Mix thoroughly by gently pipetting the entire volume up and down 10 times
- Spin down briefly in a microfuge
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Transfer 100 ng genomic DNA in 50 µl to the Covaris g-TUBE.
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Spin the g-TUBE for 1 minute at room temperature (Eppendorf 5424; 6000 rpm for 8 kb fragments).
- Spin the g-TUBE for 1 minute
- Remove and check all the DNA has passed through the g-TUBE
- If DNA remains in the upper chamber, spin again for 1 minute at the same speed
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Invert the g-TUBE and spin again for 1 minute to collect the fragmented DNA.
- Remove g-TUBE, invert the tube and replace into the centrifuge
- Spin the g-TUBE for 1 minute
- Remove and check the DNA has passed into the lower chamber
- If DNA remains in the upper chamber, spin again for 1 minute
- Remove g-TUBE
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Transfer the 50 µl fragmented DNA to a clean 1.5 ml Eppendorf DNA LoBind tube.