- Materials
-
- Rapid Adapter T (RAP T)
- Elution Buffer (EB)
- RAP Dilution Buffer (RDB) or Adapter Buffer (ADB)
- Consumables
-
- 1.5 ml Eppendorf DNA LoBind tubes
- Equipment
-
- Microfuge
- Ice bucket with ice
- P1000 pipette and tips
- P200 pipette and tips
- P100 pipette and tips
- P20 pipette and tips
- P10 pipette and tips
- P2 pipette and tips
-
Spin down the Rapid Adapter T (RAP T) and place on ice.
-
Thaw the RAP Dilution Buffer (RDB) or Adapter Buffer (ADB) at room temperature, spin down briefly using a microfuge and mix by pipetting before storing on ice.
-
In a fresh 1.5 ml Eppendorf DNA LoBind tube, dilute Rapid Adapter T (RAP T):
Reagent Volume Rapid Adapter T (RAP T) 1.2 µl RAP Dilution Buffer (RDB) or Adapter Buffer (ADB) 6.8 µl Total 8 µl -
Mix well by pipetting and spin down.
-
Add 1 µl of the diluted Rapid Adapter T (RAP T) to the amplified cDNA library, making the final volume up 24 µl.
-
Mix well by pipetting and spin down.
-
Incubate the reaction for 5 minutes at room temperature.