-
Computer specification
-
The EPI2ME platform
The EPI2ME platform (created by Metrichor Ltd., a subsidiary of Oxford Nanopore Technologies) offers a number of workflows for end-to-end analysis of nanopore data.
In a typical experiment, the user would prepare a DNA or RNA library for sequencing on a nanopore sequencing device. The sequencing experiment is started from the MinKNOW software, which controls the device and translates the electrical signals from each DNA/RNA read into basecalled sequences. The sequences for each read are saved in the .fast5 or FASTQ file format.
The basecalled FASTQ files can then be uploaded to the EPI2ME platform via a piece of software called the EPI2ME Agent. The user selects a workflow in the Agent such as 16S alignment, and the Agent transfers the read files one by one into the cloud for real-time analysis.
The analysis results are generated and updated in real-time in the form of a report in the EPI2ME platform.