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How to start sequencing
The sequencing device control, data acquisition and real-time basecalling are carried out by the MinKNOW software. It is assumed you have already installed MinKNOW on your computer. Further instructions for setting up a sequencing run can be found in the MinKNOW protocol.
We recommend setting up a sequencing run on a MinION or GridION device using the basecalling and barcoding recommendations outlined below. All other parameters can be left to their default settings.
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Open the MinKNOW software using the desktop shortcut and log into the MinKNOW software using your Community credentials.
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Click on your connected device.
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Set up a sequencing run by clicking Start sequencing.
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Select the Rapid Barcoding Kit 24 V14 (SQK-RBK114.24) or Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
Click Continue to Run Options to continue.
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Keep the run options to their default settings of 72 hour run length and 200 bp minimum read length.
Click Continue to basecalling to continue.
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Set up basecalling and barcoding using the following parameters:
Ensure basecalling is ON.
Next to "Models", click Edit options and choose High accuracy basecaller (HAC) from the drop-down menu.
Ensure barcoding in ON.
Click Continue to output and continue.
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Set up the output format and filtering as follows:
Ensure .POD5 is seleceted as the Raw reads output format.
Ensure .FASTQ is selected for basecalled reads.
Ensure filtering is ON.
Click Continue to final review to continue.
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Click "Start" to start sequencing.
You will be automatically navigated to the "Sequencing Overview" page to monitor the sequencing run.