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Optimised extraction method decision
We have developed four optimised extraction methods to generate high quality genomic DNA from your cell cultures, allowing maximised sequencing output using this method.
What extraction method is right for me?
Sample extraction method Sample type Sample Input Expected yield Expected DNA Integrity Number (DIN) Average sequencing read lengths Extraction kits used Universal bead-beating gDNA extraction Universal applications: bacteria, fungi or yeast 1 ml liquid overnight culture (~1 x 108 – 109 cfu/ml) or half of a loop of colonies from a plate >200 ng/µl per sample 7-9 ~4-7 kb QIAGEN PowerBead Tube and Promega Maxwell® RSC PureFood Pathogen kit Bacteria gDNA extraction Bacterial 200 µl liquid overnight culture (~1 x 108 – 109 cfu/ml) or 1/8 of a loop of colonies from a plate ~15-20 ng/µl per sample 9 >7 kb - Size will vary based on sample input species NEB Monarch Genomic DNA Purification Kit Hard to lyse organisms gDNA extraction Mycobacterium tuberculosis
(or hard to extract bacterial samples)5 – 10 mg cells from solid or liquid media ~15-40 ng/µl per sample 8 >7 kb - Size will vary based on sample input species NEB Monarch Genomic DNA Purification Kit Fungi gDNA extraction Fungi or yeast 2 ml of ~1 x 107 cfu/ml overnight culture or a full 10 µl inoculating loop from a plate ~40 ng/µl per sample N/A >7 kb - Size will vary based on sample input species NEB Monarch Genomic DNA Purification Kit Note: The yield, DIN and sequencing read length of extracted DNA may vary depending on sample quality and species. Please ensure you are following the correct method and using high-quality sample inputs.
Follow the links in the table above for the extraction methods documentation.
Alternatively, these extraction methods can be found in the Extraction Protocols tab in the Documentation space on the Nanopore Community