The Pre-Sequencing Mix is made up in Tube 5, containing your DNA library (Tube 4), water, Sequencing Buffer (SB) and Library Beads (LIB), which is loaded into the flow cell.

Please ensure you have completed the Flow Cell Check section before preparing your Pre-Sequencing Mix.

Transfer 37.5 µl of Sequencing Buffer (SB) into an empty 1.5 ml Eppendorf tube (Tube 5).

Resuspend the Library Beads (LIB) by gently pipetting them up and down using a P100 pipette and tip, to get a homogenous mixture.

Add 25.5 µl Library Beads (LIB) to Tube 5.

Add 12 µl of the prepared Lambda DNA library (Tube 4) to Tube 5.

• Mix the contents of the tube thoroughly by flicking the tube with your finger
• Spin down briefly in a microfuge