Defrost these at room temperature:

• Lambda DNA (LMD)
• Sequencing Buffer (SB)
• Library Beads (LIB)
• Flow Cell Flush (FCF)
• Flow Cell Tether (FCT)

Note that Fragmentation Mix (FRA), Adapter Buffer (ADB) and Rapid Adapter (RA) are not frozen.

Once the contents of each tube have thawed, mix the contents by flicking the tube, vortex the Sequencing Buffer (SB), and spin down briefly in a microfuge.

Keep the reagent tubes on ice when they are not in use.

Set up a thermal cycler with the program 30°C for 2 mins, then 80°C for 2 mins. Alternatively, set two heat blocks at 30°C and 80°C.