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6. Results and metrics
  • Introduction
  • 1. Software installation
  • 2. Hardware check
  • 3. Flow cell check
  • 4. Library preparation
  • 5. Sequencing run
  • 6. Results and metrics
  • 7. Next steps
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You are in the ‘Mac’ branch of Software installation.
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6.3

Open the EPI2ME report

Need help?

You can see further information about your run using EPI2ME. Once MinKNOW has aquired and basecalled the runs, they will be picked up by EPI2ME and sent down the chosen workflow.

For this experiment, we have already chosen the FASTQ Control Experiment workflow. This will take the basecalled reads and align them to the reference Lambda genome. You can use the report to confirm that you have produced a successful library and examine some basic genomic statistics such as depth and distribution of coverage, accuracy, etc.

Click View Report in the EPI2ME Agent window

Report view

If necessary, you may need to click Accept cookies.

The EPI2ME report will appear in a new browser window.

EPI2ME Lambda alignment

You can see your attributes (e.g. "lambda_test") have appeared at the top.
Click the Alignment_Lambda_1D tab, which will show you your reads as they appear, and map them to the lambda genome.

EPI2ME Lambda alignment2

Tip

As your base-called reads arrive from MinKNOW, they will enter the EPI2ME workflow immediately.

However, since they can take some time to pass through the workflow, there may be a slight delay before they appear on the report.

Prev 6.4 How to assess the quality of your run

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