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Example Pore-C performance
Oxford Nanopore Technologies has carried out Pore-C extractions on human cell line suspensions of NA12878, HG002/NA24385, and the cancer cell line HCC1954.
We have primarily used the 4-cutters DpnII and NlaIII in our experiments.Example read lengths of NlaIII- or DpnII-digested Pore-C extract sequencing data
Example throughput of Pore-C sequencing runs. Left: Library sequenced on a GridION with four flow cell washes. Right: Library sequenced on a PromethION with one flow cell wash. Throughput can be increased greatly with the use of flow cell washes. Please see the Library preparation section for more details.
Example Pore-C sequencing data metrics for NlaIII- or DpnII-digested Pore-C extracts.
Example Pore-C performance for NlaIII- or DpnII-digested Pore-C extracts. -
Further example data
We have released a Pore-C apps update regarding the impact of restriction enzymes on Pore-C performance and assemblies. Enzyme choice can impact the utility of Pore-C data and we have carried out four Pore-C DNA extractions in parallel using foud different restriction enzymes: the 4-cutters DpnII and NlaIII, along with the 6-cutters HindIII and SphI.