- Consumables
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- Low-lint scientific wipes (e.g. Kimberly-Clark™, cat # 7552 or equivalent)
- Double distilled water (ddH2O) (e.g. ThermoFisher, cat # 11983084)
- Freshly prepared ≥80% ethanol in nuclease-free or double distilled water, for cleaning
- ~10% Bleach (or equivalent): Thermo Scientific Alfa Aesar Sodium hypochlorite, 11-15% available chlorine, (e.g. ThermoFisher, cat # 15429019)
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Empty the tip waste container.
Dispose of the used tips in an appropriate container.
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Remove all the remaining plates from the worktable and hotel sites, and discard accordingly.
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Remove the disposable 25 ml and 100 ml troughs from the trough mounting sites, and discard accordingly.
Note: Take care not to spill any residual liquid waste when removing from the worktable.
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Remove the Sarstedt tubes from the POGO tube holder, and discard accordingly.
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Clean the Bio-Rad™ Arched Auto-Sealing Lid:
- Wipe the lid with 10% bleach
- Thoroughly rinse the bleach off the lid using ≥80% ethanol or double distilled water (ddH2O) and lint-free wipes
- Allow the lid to air dry
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Remove and/or restock the tip boxes on the worktable:
- Remove all Flexible Channel Arm (FCA) hanging tip trays from the FCA standard tip carriers, and discard accordingly.
- Remove all empty MultiChannel Arm (MCA) tip boxes from the worktable and discard accordingly.
- For partially used tip boxes, consider restacking the box with the appropriate tips for the next run.
Note: The MultiChannel Arm (MCA) tip boxes must be fully stacked. Failure to fully stack the MCA tip boxes can result in run error.
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In cases where spillage has occurred during the automated library preparation, wipe the worktable surface using ≥80% ethanol.