- Materials
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- 1200 ng gDNA per sample
- Long Fragment Buffer (LFB)
- Consumables
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- Freshly prepared 80% ethanol in nuclease-free water
- Nuclease-free water (e.g. ThermoFisher, cat #AM9937)
- Hamilton 1000 µl CO-RE tips with filter (Cat# 235905)
- Hamilton 300 µl CO-RE tips with filter (Cat# 235903)
- Hamilton 50 µl CO-RE tips with filter (Cat# 235948)
- Bio-Rad Hard-Shell® 96-Well PCR Plates (Cat# HSP9601)
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Users have the option to use pre-process to complete automated upstream methods to prepare their samples.
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Click "Pre-processes" to open the following dialogue and select the method you would like to complete and click "Ok".
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Enter the number of samples to process and click "Ok".
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Enter the input volume of your samples to process and click "Ok".
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Dialogue boxes will follow on the UI to illustrate how to correctly load the deck.