- Consumables
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- 1.5 ml Eppendorf DNA LoBind tubes
- Hamilton 50 µl CO-RE tips with filter (Cat# 235948)
- Hamilton 300 µl CO-RE tips with filter (Cat# 235903)
- Hamilton 1000 µl CO-RE tips with filter (Cat# 235905)
- Hamilton PCR ComfortLid (Cat# 814300)
- Hamilton 60 ml Reagent Reservoir, Self-Standing with Lid (Cat# 56694-01)
- Bio-Rad Hard-Shell® 96-Well PCR Plates (Cat# HSP9601)
- Equipment
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- Hamilton NGS STAR 96 (NGS STAR with Multi-Probe Head 96)
- Hamilton Ambion magnet adapter (cat# 10107866)
- Hamilton MTP landscape carrier (cat# 182365)
- Hamilton On-Deck Thermal Cycler (ODTC)
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Deck layout change
The deck layout has been updated from the standard NGS STAR 96 layout to improve the efficiency of the robot completing the protocol.
The changes will take approximately 3 minutes.
Final deck layout:
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Remove the plate stacker and two tube racks.
Plate stacker
Two tube racks
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Place both tubes racks in positions 1 and 2.
Positions 1 and 2 are indicated by the arrow below:
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Shift the four tip carriers to the left, starting from position 3.
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Place the two trough carriers next to the 300 µl tips and insert the new carrier in the remaining slot on deck.
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Place the Ambion magnet adapter on the Ambion magnet.