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Flushing and washing flow cells at high scale
This protocol is a guideline to use alongside our automation protocols for PromethION to illustrate how to load, prime and wash up to 48 flow cells on a single device. Processing multiple flow cells can be time consuming and our protocols for sequencing kits are written for individual flow cells.
We estimate ~1 hour is saved using this protocol as it minimises pipette changes, including tip and volume alterations between steps and makes the most of the incubation time by completing each step per flow cell.
The user can adapt this protocol to any number of flow cells to fit their needs.
Priming and loading 48 flow cells takes approximately 1.5-2 hours.
Washing, priming and re-loading 48 flow cells takes approximately 2-2.5 hours.For information regarding the wash kit and the nuclease activity of the Flow Cell Wash Kit, please refer to the Flow Cell Wash Kit protocol.