- Materials
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- Storage Buffer (S)
- Consumables
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- PromethION Flow Cell
- 1.5 ml Eppendorf DNA LoBind tubes
- 2 ml Eppendorf DNA LoBind tubes
- Equipment
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- P1000 pipette and tips
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Storage Buffer (S) can be used to flush flow cells for storage for later use or to check number of available nanopores before loading another library.
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Thaw Storage Buffer (S) at room temperature.
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Mix contents thoroughly by pipetting and spin down briefly.
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Slide the inlet port cover clockwise to open the inlet port of the first flow cell inserted. Repeat for all flow cells.
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After opening the inlet port, check for a small air bubble under the cover. Draw back a small volume to remove any air bubbles:
- Set a P1000 pipette to 200 µl.
- For the first flow cell, insert the tip into the inlet port.
- Turn the wheel until the dial shows 220-230 µl, or until you can see a small volume of buffer entering the pipette tip.
- Repeat for the remaining flow cells.
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Slowly add 500 μl of Storage Buffer through the inlet port of the first flow cell and repeat for the remaining flow cells.
After each row (8 flow cells), thoroughly mix the Storage Buffer by pipetting.
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Once all the flow cells are loaded, close the inlet port covers, and remove any buffer from the waste port.
The waste liquid can be aspirated from either one of the ports, labelled 2 and 3 on the flow cell.
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Remove the flow cells from the device by pressing down on the latch to release the flow cells before sliding out smoothly.
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Store the flow cells at 4-8° C.