- Materials
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- Elution Buffer from the Oxford Nanopore kit (EB)
- Rapid Adapter T (RAP T)
- Consumables
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- 1.5 ml Eppendorf DNA LoBind tubes
- Equipment
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- Microfuge
- Ice bucket with ice
- P1000 pipette and tips
- P200 pipette and tips
- P100 pipette and tips
- P20 pipette and tips
- P10 pipette and tips
- P2 pipette and tips
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Analyse 1 µl of sample using the Agilent Bioanalyzer. Determine the average amplicon size from this data, and use this to calculate the input sample volume for the next step.
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Calculate the required sample volume for 35 fmol and dilute this into 12 μl of EB.
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Add 0.5 μl of Rapid Adapter T (RAP T) to the amplified cDNA library.
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Mix well by pipetting and spin down.
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Incubate the reaction for 5 minutes at room temperature.