Overview
- We advise all customers to read and consider the information in the "Targeted, amplification-free DNA sequencing using CRISPR/Cas" info sheet before starting this protocol
- This protocol uses genomic DNA
- Targeted cutting around specific genomic Regions of Interest (ROI) to achieve enrichment
- Library preparation time is ~110 minutes
- Fragment lengths are determined by the cut spacing, and not fragmentation
- No PCR is required
For Research Use Only
This is a Legacy product
This kit is soon to be discontinued. If customers require further support for any ongoing critical experiments using a Legacy product, please contact Customer Support via email: support@nanoporetech.com.
Introduction to the protocol
Library preparation
Sequencing and data analysis
Troubleshooting