Products

Discover nanopore sequencing

What can it do? How does it work? Our platform performance and accuracy

Explore products

Prepare Sequence Analyse
Applications

Focus areas

From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.

Research

Microbial genomics Plant Human genomics Animal Cancer research Microbiome Infectious disease Population genomics Clinical research Environmental research & conservation

Techniques

Whole-genome sequencing Epigenetics Transcriptomics SNVs and phasing Multiomics Assembly Metagenomics Fusion transcripts Targeted sequencing Gene expression Single cell & spatial transcriptomics Splice variation Structural variation Chromatin conformation

Focus areas

Biopharma Education Clinical
Store Resources

Documentation

Find all the documentation needed for your nanopore experiments, including protocols and device manuals.

View all documentation

Nanopore Learning

Explore our online courses and video lessons to support your nanopore sequencing journey.

View Nanopore Learning

Resources

Documentation Publications Case studies Videos Posters Brochures White papers Workflows Getting started guides Application notes View all resources
Support About

Company

About us Board members Executive team Investor centre Company history Sustainability Careers Media resources

News & Events

News Events Blogs

Global partners

Channel partners Sequencing service providers Compatible products For partners
Oxford Nanopore Technologies Fully scalable, real-time DNA/RNA sequencing technology Oxford Nanopore Diagnostics

London Calling 2025

Submit an abstract
Dashboard Getting started Posts Groups
Nanopore Learning Documentation Highlights Updates Profile
Back
Nanopore Learning / Courses /
Human genome sequencing and analysis

In this course, we will introduce the latest updates and end-to-end workflows for sequencing the Human genome using the Kit V14 chemistry and performing analysis using the wf-human-variation workflow. This course will introduce and discuss kit V14 library preparation options, sequencing set up and analysis through the EPI2ME platform for the detection of SNPs, indels, SVs and methylation.

0 % Complete
Course overview

Human Genomics Planning

1 Introduction to Sequencing Human DNA with Nanopore
2 Introduction to sequencing devices
3 P2 Solo: First-time setup and hardware check
4 Library preparation: Extraction methods and sample handling
5 Library preparation: Quality control of input DNA or RNA
6 Improving sequencing yield

Human Genomics Preparation

7 Library preparation: Ligation sequencing kit
8 Kit V14 introduction and overview
9 Kit V14 performance
10 MinKNOW: The graphical user interface (GUI)
11 Flow cell handling

Human Genomics Sequencing

12 A practical demonstration of priming and loading a flow cell
13 MinKNOW: Setting up a new experiment
14 MinKNOW Configuration for Kit 14
15 Methylation Detection On MinKNOW
16 MinKNOW: Live basecalling and output folder structure
17 MinKNOW: Assessing your run
18 Introduction: Real-time analysis

Human Genomics Analysis

19 Introduction to data generation and sequencing output for analysis
20 Introduction to data analysis solutions
21 Methylation Detection: Sample to Answer Workflow Overview
22 Data Analysis: EPI2ME Labs introduction
23 EPI2ME Labs: wf-human-variation Platform Demo
24 Introduction to EPI2ME Labs workflows
25 EPI2ME Labs Nextflow Workflows demonstration
26 Introduction to SNP and indel detection
27 Introduction to structural variation detection

Improving sequencing yield

8 min
Learning objectives
  • Learning how to keep the pores occupied
  • Considerations for long or short fragment libraries and starting input
  • Size selection considerations
Related content

The links below are outside this course and will open in a new tab.

Lesson library
Links

What are the benefits of community membership?

Posts

Interact with thousands of other nanopore sequencers from around the globe.

Private Channels

Private channels are available to discuss specific topics and technology applications.

Product and technology updates

Stay up to date with the latest Oxford Nanopore products and technology.

Software

Download latest versions of sequencing and analysis software.

 Login
@2008 - 2024 Oxford Nanopore Technologies. All rights reserved

Cookies Notice

We use cookies and similar technologies on our websites to help provide you with the best possible online experience.

By using our sites and apps, you agree that we may store and access cookies and similar technologies on your device.