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For more information about setting up your P2 Solo, refer to our Nanopore Learning video:
P2 Solo: First-time setup and hardware check
Our P2 Solo FAQs are located here.
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Recommended laboratory location
- Place the P2 Solo and GridION devices on a strong, clean laboratory bench
- Ensure power connectors are in close proximity
- Ensure power socket is easily accessible should a disconnection be required in case of emergency
- Sequencing should be carried out at a room temperature of +18°C to +22°C with device lid closed prior to sequencing beginning
- Allow 30 cm clearance to rear and sides of the device
- Do not cover any ventilation grills
- WARNING - The rear of the device will heat up during operation
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Check that your GridION™ serial number is greater than GXB002xxx.
The serial number can be found on the sticker on the back of the GridION. Ensure your GridION is powered on and check your GridION is updated to the latest MinKNOW™ version that is compatible with the P2 Solo (see the Software Downloads page on the Nanopore Community). Please read the IT requirements document for full details. Launch MinKNOW by clicking on the Nanopore Wheel icon on your desktop.
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Unpack the PromethION 2 Solo device and place it on the bench where it is to be run.
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Download the PromethION 2 Solo software.
For Windows and Mac computers, navigate to the Software Downloads page of the Nanopore Community.
For Linux, see the instructions in this section for how to install the software via the Terminal. -
Ensure there are no flow cells or CTCs inserted into the P2 Solo.
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Plug the P2 Solo AC power adapter into a wall socket and the plug the power cable into the P2 Solo.
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Plug one end of the USB Type-C to Type-C cable into the USB Type-C port on the GridION (see diagram) and the other end into P2 Solo USB Type-C port. Make sure you can feel and hear the USB-C connection click in on the P2 Solo and GridION. You may need to pull down the metal tabs on either side of the USB-C connector on the GridION to fully insert. Toggle the power switch to turn on the device. Wait up to 60 seconds for the devices to be initialised in MinKNOW. The front LEDs will light up once complete.
For more information about how to correctly connect your PromethION 2 Solo to the GridION via the USB Type-C cable, refer to this FAQ:
I'm unable to connect my P2 Solo to MinKNOW -
We recommend users to log into the MinKNOW software using their Community credentials.
To log in, you must be connected to the internet.
From here, you can also change the language in the user interface in the bottom right corner.
You will be prompted to enter your email address and Nanopore password:
If you experience login issues, please visit the Community Support channel (https://community.nanoporetech.com/support) and use Continue as guest for temporary use.
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Select the sequencing device in the Connection Manager to connect to the computer.
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You will be taken to the sequencing overview page.