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For more information about setting up your P2 Solo, refer to our Nanopore Learning video:
P2 Solo: First-time setup and hardware check
Our P2 Solo FAQs are located here.
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Recommended laboratory location
- Place the P2 Solo device on a clean laboratory bench
- Ensure power connectors are in close proximity
- Ensure power socket is easily accessible should a disconnection be required in case of emergency
- Sequencing should be carried out at a room temperature of +18°C to +22°C with device lid closed prior to sequencing beginning
- Allow 30 cm clearance to rear and sides of the device
- Do not cover any ventilation grills
- WARNING - The rear of the device will heat up during operation
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Make sure the compute meets the IT requirements set out in the P2 Solo IT Requirements document.
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Downloading and installing MinKNOW
The MinKNOW software for the PromethION 2 Solo is available to download from the Software Downloads page on the Community.
All Oxford Nanopore devices use MinKNOW™ as the primary software. The MinKNOW software carries out several core tasks: data acquisition, real-time analysis and feedback, basecalling, data streaming, controlling the device, and ensuring that the platform chemistry is performing correctly to run the samples. MinKNOW takes the raw data and converts it into reads by recognition of the distinctive change in current that occurs when a DNA strand enters and leaves the pore. MinKNOW then basecalls the reads, and writes out the data into POD5 or FASTQ files.
Please follow the MinKNOW protocol for information on how to install the software.
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Unpack the PromethION 2 Solo device and place it on the bench where it is to be run.
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Download the PromethION 2 Solo software.
For Windows and Mac computers, navigate to the Software Downloads page of the Nanopore Community.
For Linux, see the instructions in this section for how to install the software via the Terminal. -
Ensure there are no flow cells or CTCs inserted into the P2 Solo.
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Plug the P2 Solo AC power adapter into a wall socket and the plug the power cable into the P2 Solo.
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Plug one end of the USB Type-C to Type-C cable into the USB Type-C port on the laptop and the other end into P2 Solo USB Type-C port. Make sure you can feel and hear the USB-C connection click in on the P2 Solo and your workstation. Toggle the power switch to turn on the device. Wait up to 60 seconds for the devices to be initialised in MinKNOW. The front LEDs will light up once complete.
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Double-click the MinKNOW icon located on the desktop to open the MinKNOW UI.
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We recommend users to log into the MinKNOW software using their Community credentials.
To log in, you must be connected to the internet.
From here, you can also change the language in the user interface in the bottom right corner.
You will be prompted to enter your email address and Nanopore password:
If you experience login issues, please visit the Community Support channel (https://community.nanoporetech.com/support) and use Continue as guest for temporary use.
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Select the sequencing devices in the Connection Manager to connect to the computer.
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You will be taken to the sequencing overview page.