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Intended use of the MinION Mk1B and Mk1C
Oxford Nanopore Technologies® MinION device is an electronic analysis system for use in scientific research. The core technology is built around a nanopore that is able to detect single molecule events including nucleic acids (DNA/RNA), proteins and small molecules.
It is for research use only and not for in vitro diagnostic or therapeutic use.
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The purpose of the safety information
The safety information provides you with the details needed to install and use the system safely.
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MinION Mk1B electrical output values
Supply voltage 5 V Operating current 800 mA for 10 k
900 mA for 33 k
1 A maximumMaximum power 5 W -
MinION Mk1C electrical output values
Power supply 6.3-19.6 VDC Max rated current 10 A Max rated power 60 W -
Safety notices
WARNING: indicates a hazardous situation which, if not avoided, could result in death or serious injury. It is important not to proceed until all stated conditions are met and clearly understood.
CAUTION: indicates a hazardous situation which, if not avoided, could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
ADVISORY: indicates instructions that must be followed to avoid damage to the product or other equipment.
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Material Safety Data Sheets (MSDSs)
The latest versions of the Material Safety Data Sheets are available in the Nanopore Store. Each product has a Safety and Legal tab, and the MSDSs are saved here and can be downloaded as required. For further assistance, please contact support@nanoporetech.com .
Read and understand the MSDSs before handling, working with or storing the chemicals being used within the Oxford Nanopore Technologies® sequencing device.
Minimise contact with the chemicals by wearing protective clothing, safety glasses and gloves. The MSDS will carry specific requirements.
Minimise inhalation of chemicals by using appropriate and adequate ventilation. The MSDS will carry any specific requirements.
Continuously check for any spills or leakages. If a spill or leak occurs, follow the cleanup guidelines provided on the MSDS.
All components of the MinION devices system should be handled, stored and disposed of in accordance with local, state/provincial or national laws and regulations.
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General precautions
When handling toxic, radioactive or pathogenic samples as defined by the WHO Laboratory Biosafety Manual, observe the safety regulations of the specific local in question.
Do not use the MinION device if it has suffered any damage, e.g. to power cables, data transfer cables, power supplies, or flow cells.
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Personal protection
Specimens and reagents containing materials from humans should be treated as potentially infectious. Use safe laboratory procedures as outlined in publications such as Biosafety in Microbiological and Biomedical Laboratories (http://www.cdc.gov/biosafety/publications/bmbl5/index.htm).
The operator has to take all necessary actions to avoid spreading hazardous biological agents in the vicinity of the system. The facility should comply with the national code of practice for biosafety.
Samples being loaded into the flow cell should be used, stored and disposed of according to the required safety regulations and laws. Consult the responsible body for safety in your lab for local regulations.
Samples containing infectious agents should be handled with the greatest of care and in accordance with the required safety regulations and laws.
It is good laboratory practice to always wear safety glasses, gloves (two pairs if working with infectious agents) and a lab coat. There may be other locally advised items which add to this recommendation.
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Use of the MinION Mk1B and Mk1C, flow cells and reagents
When handling toxic, radioactive or pathogenic samples as defined by the WHO Laboratory Biosafety Manual, observe the specific local regulations.
Loading excess buffer, sample or de-ioinised water to the Flow Cell will cause an overflow of the waste compartment. Absorbent material should be used to capture sample and buffer which will come out through the waste port. All material should be disposed of in line with local regulations for biological waste.
In the unlikely event that the sequencing device is found to be hot during use, disconnect the device from the host computer and refer to troubleshooting guidelines.
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Maintenance
Repairs must only be performed by Oxford Nanopore Technologies, and no components should be replaced. Contact support@nanoporetech.com in the event of damage to the sequencing device or flow cells.
Before using cleaning or decontamination methods other than those stipulated by the manufacturer, contact the manufacturer to ensure that the intended method will not damage the sequencing device and/or flow cell.
When returning sequencing devices and/or flow cells, ensure that they are fully decontaminated and do not present any health risk to our staff.
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Disposal and recycling instructions
Used plasticware, such as reagents, tubes and pipette tips, must be collected and disposed of properly in accordance with local safety regulations and laboratory procedures.
The flow cell buffer, wash kit buffers, and library preparation kit buffers must not be mixed and must be kept away from strong acids and alkalis.
The flow cell buffer, wash kit buffers, and library preparation kit buffers must be disposed of according to the local regulations. They must not be disposed of down a sink.
The Terms and Conditions for the use of sequencing devices stipulate that any flow cells that have been used with or otherwise been in contact with materials of Biohazard Level 3 or higher (“ Contaminated Flow Cells ”) must not be returned. Proof of legal and appropriate destruction of any contaminated Flow Cells will be required.
The sequencing device shall be decontaminated before decommissioning, and all local regulations for electronic and electrical waste shall be followed with regard to disposal of the components if they are not being returned to Oxford Nanopore Technologies.
The flow cell shall be disposed of as hazardous biological waste, and all local regulation for such waste shall be followed if they are not being returned to Oxford Nanopore Technologies.
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Labels on the instrument
Label on the MinION Mk1B:
Label on the SpotON Flow Cell: