Guide - MinION - Gsr_9203_v1_reva_19jan2024- 17/32

5

Sequencing run

Approx 40 Mins

Goal: to prime your flow cell with buffer, load your RNA library into the flow cell, and start a sequencing experiment in MinKNOW.

This step will take you through flow cell priming, sample loading and starting your sequencing experiment.

What you will need

1.5 ml Eppendorf DNA LoBind tubes
Control RNA library
Flow Cell Flush (FCF)
Flow Cell Flush (FCF)
Flow cell, MinION Mk1B, USB3 cable and compatible computer - set up in previous sections
Library Solution (LIS)
Microfuge
MinION Flow Cell Light Shield
Nuclease-free water
P100 pipette and tips
P100 pipette and tips
P1000 pipette and tips
P1000 pipette and tips
P20 pipette and tips
RNA Flush Tether (RFT)
RNA Flush Tether (RFT)
Sequencing Buffer (SB)
Vortex mixer

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