We aim to walk you through the set up of your MinION Mk1B and your control sequencing experiment.

Before you start sequencing your own RNA samples, we recommend that you sequence a control sample of the yeast enolase-2 transcript, which is supplied in your sequencing kit. Sequencing a control sample allows you to practise performing an end-to-end nanopore sequencing experiment and to become familiar with the technology.

The whole set up process typically takes 4 hours. Once started, the sequencing run takes 6 hours, but can be left unattended.

If at any point you get stuck, look out for explainers and FAQs to help you.