Make sure the contents of the Flow Cell Flush (FCF) and RNA Flush Tether (RFT) tubes are fully defrosted. Mix the tubes by vortexing and then spin down in a microfuge.

Take a clean 1.5 ml Eppendorf DNA LoBind tube (Tube 6) and add 1,170 µl of Flow Cell Flush (FCF).

Add 30 µl of RNA Flush Tether (RFT) to Tube 6.

Mix the contents of the tube by vortexing and then spin down briefly in a microfuge.