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Nanopore Learning / Courses /
Control Experiment: Ligation sequencing guide

This course provides guidance for running a control experiment on a MinION using the Ligation Sequencing Kit

0 % Complete
Course overview

Planning

1 DNA (Lambda) Control Experiment Overview
2 MinION introduction
3 Flow cell introduction
4 Flow cell handling

Preparation

5 MinKNOW: Introduction
6 EPI2ME Introduction
7 MinKNOW: Installation
8 EPI2ME Installation
9 MinKNOW: Hardware check
10 MinKNOW: Flow cell check

Sequencing

11 Ligation Lambda Control Experiment
12 Library preparation: Ligation sequencing kit
13 Priming and loading your flow cell
14 MinKNOW: Setting up a new experiment

Analysis

15 Lambda Control Experiment Run Metrics and Results
Course completion

Priming and loading your flow cell

7 min
Learning objectives
  • Good practices for priming and loading the flow cell
  • How to effectively remove air pockets within the flow cell
  • The dangers of incorrectly loading the flow cell
  • How to correctly load the flowcell
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