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Nanopore Learning / Courses /
Control Experiment: Ligation sequencing guide

This course provides guidance for running a control experiment on a MinION using the Ligation Sequencing Kit

0 % Complete
Course overview

Planning

1 DNA (Lambda) Control Experiment Overview
2 MinION introduction
3 Flow cell introduction
4 Flow cell handling

Preparation

5 MinKNOW: Introduction
6 EPI2ME Introduction
7 MinKNOW: Installation
8 EPI2ME Installation
9 MinKNOW: Hardware check
10 MinKNOW: Flow cell check

Sequencing

11 Ligation Lambda Control Experiment
12 Library preparation: Ligation sequencing kit
13 Priming and loading your flow cell
14 MinKNOW: Setting up a new experiment

Analysis

15 Lambda Control Experiment Run Metrics and Results
Course completion

MinION introduction

5 min
Learning objectives
  • What’s in the box
  • The function of the components of the Minion device
  • Applications of Minion sequencing
  • Real time data acquisition, IT requirements and throughput
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