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Nanopore Learning / Courses /
Control Experiment: Rapid sequencing guid

This course provides guidance for running a control experiment on a MinION using the Rapid Sequencing Kit

0 % Complete
Course overview

Planning

1 DNA (Lambda) Control Experiment Overview
2 MinION introduction
3 Flow cell introduction
4 Flow cell handling

Preparation

5 MinKNOW: Introduction
6 MinKNOW: Installation
7 MinKNOW: Hardware check
8 MinKNOW: Flow cell check

Sequencing

9 Rapid Lambda Control Experiment
10 Library preparation: Rapid sequencing kits
11 Priming and loading your flow cell
12 MinKNOW: Setting up a new experiment

Analysis

13 MinKNOW: Assessing your run
14 MinKNOW: Pore occupancy
Course completion

MinKNOW: Pore occupancy

6 min
Learning objectives
  • Understand pore occupancy
  • Monitor and interpret the duty time plot
  • Learn to troubleshoot your run in real time
  • Monitor and interpret the channels panel
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