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Flow cell check
We strongly recommend performing a flow cell check before loading a DNA or RNA library to assess the number of pores available.
Oxford Nanopore Technologies will replace any flow cell that falls below the warranty number of active pores within three months of purchase, provided that you report the results within two days of performing the flow cell check and you have followed the storage recommendations.
Flow cell Minimum number of active pores covered by warranty Flongle Flow Cell (FLO-FLG001) 50 MinION/GridION Flow Cell 800 PromethION Flow Cell 5000 -
GridION X5 LEDs
When a flow cell is inserted into a device, the flow cell position indicator LED will light up a colour depending on the condition of the ASIC on the flow cell. The application-specific integrated circuit (ASIC) is connected to the sensor array and measures the ionic current flow across the nanopores.
- Blue: No ASIC is detected on the flow cell
- Green: ASIC is detected
- Red: There is a problem with the ASIC
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When you see the flow cell type and the flow cell ID is recognised, click 'Start' to begin.
This example is on a GridION device:
Note: For Flongle, the flow cell ID must be filled in manually.
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Flow cell health indicators
The quality of the flow cell will be shown as one of the three outcomes:
Yellow exclamation mark (Flongle flow cell): The number of sequencing pores is below warranty. Take the flow cell out of the device, re-insert it and run a flow cell check again. If the flow cell is still below warranty, contact support@nanoporetech.com
Green tick (MinION flow cell): The number of sequencing pores is above warranty.
Question mark (PromethION flow cell): A flow cell check has not been run on the flow cell during this MinKNOW session.
Note: The indicator of quality will only remain visible during a MinKNOW session. Once the MinKNOW session has ended, the status of the flow cell will be erased.