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Introduction to MinKNOW
The MinKNOWâ„¢ software carries out several core tasks: data acquisition, real-time analysis and feedback, basecalling, data streaming, controlling the device, and ensuring that the platform chemistry is performing correctly to run the samples. MinKNOW takes the raw data and converts it into reads by recognition of the distinctive change in current that occurs when a DNA strand enters and leaves the pore. MinKNOW then basecalls the reads, and writes out the data into .fast5 or FASTQ files.
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MinKNOW for MinION, GridION and PromethION
All Oxford Nanopore devices use MinKNOW as the primary software. The user interface differs depending on the device, however the underlying instrument control and data acquisition functions are the same.