Load your flow cell
Pipetting tips
The following section will load fluid and sample into the flow cell. In the images below are some simple tips on pipetting technique which are essential to avoid introducing air bubbles and damaging the sensor array.
When drawing up solution:
- make sure there is no air plug at the bottom of the tip
When ejecting the solution:
- do not fully expel the liquid from the tip
- leave a small volume in the tip end so that no air can follow the solution into the inlet port
Open the priming port by sliding the cover clockwise so that the port is visible.
Open the sample port by gently lifting the sample port cover. You will still need to use the priming port, so keep this open.
Using a P1000 pipette, slowly load 200 µl of the Priming Mix into the priming port.
Take care to not introduce air bubbles. Pipette slowly, to avoid the buffer bubbling out of the sample port.
Carry out this step immediately before loading the library.
Using a P200 pipette, hold the pipette tip just above the sample port and slowly load 75 µl, drop by drop, of the Pre-Sequencing Mix (Tube 5).
- Close the sample port with the cover ensuring the bung enters into the port
- Close the priming port
- Close the MinION Mk1B lid
Place the light shield onto the flow cell. Carefully place the leading edge of the light shield against the clip. Do not force the light shield underneath the clip. Gently lower the light shield onto the flow cell. The light shield should sit around the SpotON cover, covering the entire top section of the flow cell.
