The pre-sequencing mix is a mix of your RNA library, Sequencing Buffer (SB) and Library Solution (LIS) that is loaded into the flow cell.

Please ensure you have completed the Flow Cell Check section before preparing your pre-sequencing mix.

Take the 1.5 ml tube containing your control RNA library and add 37.5 µl Sequencing Buffer (SB).

Add 25.5 µl RNA Library Solution (LIS) to the tube.

• Mix the contents of the tube thoroughly by flicking the tube with your finger
• Spin down briefly in a microfuge