- Materials
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- VolTRAX Configuration Kit (VCK-V2003b)
- VolTRAX Sequencing Kit (VSK-VSK004)
- VolTRAX Multiplex Kit (VSK-VMK004)
- VolTRAX PCR Tiling 1-12 COVID-19 Kit (VSK-PTC001)
- VolTRAX RT-PCR Sequencing Kit 1-12 (VSK-VPS001)
- VolTRAX Cartridge Pack (VCT-V2002B)
- Consumables
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- VolTRAX cartridge
- MinION Flow Cell
- 1.5 ml Eppendorf DNA LoBind tubes
- 0.2 ml thin-walled PCR tubes
- Equipment
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- VolTRAX V2b
- P20 pipette and tips
- P10 pipette and tips
- MinION
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Sample requirements to prepare high-quality libraries (see individual kit sections for details)
The VolTRAX Sequencing Kit (VSK-VSK004) and VolTRAX Multiplex Kit (VSK-VMK004) protocols for VolTRAX V2b require clean, extracted DNA that is of high molecular weight, accurately-quantified and free from contaminants and detergents. The DNA should be prepared in 10 mM Tris-HCl, pH 8.0.
The VolTRAX PCR Tiling 1-12 COVID-19 Kit (VSK-PTC001) and VolTRAX RT-PCR Sequencing Kit 1-12 protocol requires total RNA in 10 mM Tris-HCl, pH 8.0, extracted from samples that have been screened by a suitable qPCR assay. Samples must have a Ct value of 30 or less, and be free from contaminants and detergents.
Contaminants
Contaminants can impede the function of the library preparation chemistry for VolTRAX V2b. For information about the impact of contaminants on reaction efficiency, see the Contaminants Know-How piece.