- Materials
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- VolTRAX Multiplex Kit (VSK-VMK004)
- VolTRAX Cartridge Pack (VCT-V2002B)
- 1.8 μl of extracted gDNA in 10 mM Tris-HCl, pH 8.0 at a DNA concentration of 50 ng/μl (90 ng total) for each sample to be barcoded
- Consumables
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- 0.2 ml thin-walled PCR tubes
- Qubit dsDNA HS Assay Kit (ThermoFisher, cat # Q32851)
- Equipment
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- VolTRAX V2b
- P20 pipette and tips
- P2 pipette and tips
- Qubit fluorometer (or equivalent for QC check)
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Start the VolTRAX software. You will be presented with the following options for protocols to run. Select "VolTRAX Multiplex Kit" to start the protocol and follow the instructions in the software.
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Protocol preview
Once an option is selected from the menu, the UI will show a preview of the steps involved in library preparation. The example below is for the Configuration Kit.
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When prompted by the UI, connect your VolTRAX device to the computer. When it is connected, you will then be prompted to insert a cartridge into the device.
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You will be presented with five options for test experiments to run. Select "Multiplexing Kit" to start the workflow.
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Insert the cartridge firmly into the device until you hear a click. From here on, do not remove the cartridge until the end of the experiment.
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Prime the cartridge as instructed in the UI by twisting the Priming Fluid container anti-clockwise and removing the foil tab to allow the fluid to enter the cartridge.
Select Continue when each step is completed.
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Select the number of samples you are preparing and assign sample aliases to barcodes.
Once you have entered the required details, click Confirm. You will be taken to the Sample Label Check page where you can confirm that the details are correct.
If you are running fewer than 10 samples, you can adjust the "Sample number". Add VBS (VolTRAX Buffer Solution) to wells that will be without a sample.
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Wait for the library prep to finish.
A timer can be found on the right hand side of the UI, indicating the remaining time.
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At the end of the experiment, extract the prepared library from the E port of the VolTRAX cartridge. Dispense the library into a 0.2 ml PCR tube for preparation of the sequencing mix, as discussed in the following section.
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Optional actionQuantify 1 µl of eluted sample using a Qubit fluorometer.