- Materials
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- 9.5 μl DNA in 10 mM Tris-HCl, pH 8.0 at a DNA concentration of 50 ng/μl (475 ng total)
- VolTRAX Sequencing Kit (VSK-VSK004)
- VolTRAX Cartridge Pack (VCT-V2002B)
- Consumables
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- 0.2 ml thin-walled PCR tubes
- Qubit dsDNA HS Assay Kit (ThermoFisher, cat # Q32851)
- Equipment
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- VolTRAX V2b
- P20 pipette and tips
- P10 pipette and tips
- Optional equipment
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- Qubit fluorometer (or equivalent for QC check)
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Start the VolTRAX software. You will be presented with the following options for protocols to run. Select "Sequencing Kit" to start the protocol and follow the instructions in the software.
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Protocol preview
Once an option is selected from the menu, the UI will show a preview of the steps involved in library preparation. The example below is for the Configuration Kit.
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When prompted by the UI, connect your VolTRAX device to the computer. When it is connected, you will then be prompted to insert a cartridge into the device.
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Prime the cartridge as instructed in the UI by twisting the Priming Fluid container anti-clockwise and removing the foil tab to allow the fluid to enter the cartridge.
Select Continue when each step is completed.
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Wait for the library prep to finish.
A timer can be found on the right hand side of the UI, indicating the remaining time.
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At the end of the experiment, extract the prepared library from the E port of the VolTRAX cartridge. Dispense the library into a 0.2 ml PCR tube for preparation of the sequencing mix, as discussed in the following section.
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Quantify 1 µl of eluted sample using a Qubit fluorometer.