- Materials
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- VolTRAX Configuration Kit (VCK-V2003b)
- Equipment
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- VolTRAX V2b
- P20 pipette and tips
- P10 pipette and tips
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The VolTRAX Configuration Kit (VCK-V2003b) enables the practice of loading and extracting material from the VolTRAX V2b cartridge.
Below is an overview of the configuration experiment using a VolTRAX V2b with the Configuration Kit (VCK-V2003b).
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An overview of VolTRAX V2b configuration
1. Loading a protocol
- Use the protocol selector in the UI to load the VCK-V2003b protocol.
- When prompted, connect your VolTRAX V2b device, press Continue and await connection.
2. System QC - Cartridge Test
- When prompted, connect a cartridge to the VolTRAX device and press Continue to proceed to the Cartridge Test.
3. Running and loading VCS
- Follow instructions and advice on the equipment required and pipetting technique
- Prime the cartridge as instructed by the UI
- Load the VolTRAX Configuration Solution (VCS) and the VX AMPure XP Config (VXC) into the ports as instructed by the UI
- The solutions will be manipulated to indicate what the user should expect during a run
4. Extracting the VolTRAX Configuration Solution from the cartridge
- Follow the extraction instructions, using UI as a visual guide, to slowly extract the VCS
- Discard the extracted VCS
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Start the VolTRAX software. You will be presented with the following options for protocols to run. Select "Configuration Kit" to start the protocol and follow the instructions in the software.
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Protocol preview
Once an option is selected from the menu, the UI will show a preview of the steps involved in library preparation. The example below is for the Configuration Kit.
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When prompted by the UI, connect your VolTRAX device to the computer. When it is connected, you will then be prompted to insert a cartridge into the device.
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Prime the cartridge as instructed in the UI by twisting the Priming Fluid container anti-clockwise and removing the foil tab to allow the fluid to enter the cartridge.
Select Continue when each step is completed.
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Follow the steps as described in the UI, loading VolTRAX Configuration Solution and VX AMPure XP Config into the wells instead of the reagents stated.
Select Continue after each step. After all the steps have been completed, the library prep process will start automatically.