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The VSK-VSK004 DNA library preparation kit
The VolTRAX V2b provides an automated process for preparing a DNA library. The chemistry of the library preparation is the same as for manual library preparation for a MinION or GridION sequencing experiment.
Below is an overview of the library preparation stages using a VolTRAX V2b, blue cartridge and the VolTRAX Sequencing Kit (VSK-VSK004).
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Overview of the VolTRAX Sequencing Kit protocol
1. Loading a protocol
- Use the protocol selector in the UI to load the VSK-VSK004 protocol.
- When prompted, connect your VolTRAX V2b device, press Continue and await connection.
2. System QC - Cartridge Test
- When prompted, connect a cartridge to the VolTRAX device and press Continue to proceed to the Cartridge Test.
3. Loading the Priming Fluid and reagents into the cartridge
- Prime the cartridge by twisting the oil container and removing the foil tab.
- The UI will recommend the quality, concentration, buffer and Absorbance (A) readings for your input DNA. These are stated at the stage of 'Reagent Loading' later in this protocol.
- Follow the instructions on the equipment required and pipetting technique.
- Load the appropriate reagents and extracted DNA into the ports as instructed by the UI.
- The reagents will be manipulated by the VolTRAX V2b to prepare the library for nanopore sequencing.
4. Extracting the library from the cartridge
- Follow the extraction instructions, using UI as a visual guide, to slowly extract the library from the cartridge, then transfer it into an Eppendorf tube.
- Prepare the library for loading onto the flow cell.
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VolTRAX Sequencing Kit (VSK-VSK004) contents
Name Acronym Cap colour Number of vials Fill volume (μl) VX Fragmentation Mix VFA Amber 1 25 EDTA EDTA Clear 1 20 VX AMPure XP Beads VXP Pink 1 45 VX Long Fragment Buffer VLF Orange 1 40 VX Rapid Adapter T VRA T Green 1 25 VX Elution Buffer VEB Black 1 65 VX Running Buffer VRB Red 1 250 Flush Buffer FB Blue 3 1,170 Flush Tether FLT White cap, purple label 1 200 -
VSK-VSK004 chemistry
The VSK-VSK004 library preparation protocol applies our rapid chemistry, with a bead-based purification step.