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Native barcode sequences
Below is the full list of our native barcode (NB01-96) sequences. The first 24 unique barcodes are available in the Native Barcoding Kit 24 V14 (SQK-NBD114.24). The Native Barcoding Kit 96 V14 (SQK-NBD114.96) include the first 24 native barcodes, with the additional 72 unique barcodes. The native barcodes are shipped at 640 nM.
In addition to the barcodes, there are also flanking sequences which add an extra level of context during analysis.
Barcode flanking sequences:
Forward sequence: 5' - AAGGTTAA - barcode - CAGCACCT - 3'
Reverse sequence: 5' - GGTGCTG - barcode - TTAACCTTAGCAAT - 3'Native barcode sequences
Component Forward sequence Reverse sequence NB01 CACAAAGACACCGACAACTTTCTT AAGAAAGTTGTCGGTGTCTTTGTG NB02 ACAGACGACTACAAACGGAATCGA TCGATTCCGTTTGTAGTCGTCTGT NB03 CCTGGTAACTGGGACACAAGACTC GAGTCTTGTGTCCCAGTTACCAGG NB04 TAGGGAAACACGATAGAATCCGAA TTCGGATTCTATCGTGTTTCCCTA NB05 AAGGTTACACAAACCCTGGACAAG CTTGTCCAGGGTTTGTGTAACCTT NB06 GACTACTTTCTGCCTTTGCGAGAA TTCTCGCAAAGGCAGAAAGTAGTC NB07 AAGGATTCATTCCCACGGTAACAC GTGTTACCGTGGGAATGAATCCTT NB08 ACGTAACTTGGTTTGTTCCCTGAA TTCAGGGAACAAACCAAGTTACGT NB09 AACCAAGACTCGCTGTGCCTAGTT AACTAGGCACAGCGAGTCTTGGTT NB10 GAGAGGACAAAGGTTTCAACGCTT AAGCGTTGAAACCTTTGTCCTCTC NB11 TCCATTCCCTCCGATAGATGAAAC GTTTCATCTATCGGAGGGAATGGA NB12 TCCGATTCTGCTTCTTTCTACCTG CAGGTAGAAAGAAGCAGAATCGGA NB13 AGAACGACTTCCATACTCGTGTGA TCACACGAGTATGGAAGTCGTTCT NB14 AACGAGTCTCTTGGGACCCATAGA TCTATGGGTCCCAAGAGACTCGTT NB15 AGGTCTACCTCGCTAACACCACTG CAGTGGTGTTAGCGAGGTAGACCT NB16 CGTCAACTGACAGTGGTTCGTACT AGTACGAACCACTGTCAGTTGACG NB17 ACCCTCCAGGAAAGTACCTCTGAT ATCAGAGGTACTTTCCTGGAGGGT NB18 CCAAACCCAACAACCTAGATAGGC GCCTATCTAGGTTGTTGGGTTTGG NB19 GTTCCTCGTGCAGTGTCAAGAGAT ATCTCTTGACACTGCACGAGGAAC NB20 TTGCGTCCTGTTACGAGAACTCAT ATGAGTTCTCGTAACAGGACGCAA NB21 GAGCCTCTCATTGTCCGTTCTCTA TAGAGAACGGACAATGAGAGGCTC NB22 ACCACTGCCATGTATCAAAGTACG CGTACTTTGATACATGGCAGTGGT NB23 CTTACTACCCAGTGAACCTCCTCG CGAGGAGGTTCACTGGGTAGTAAG NB24 GCATAGTTCTGCATGATGGGTTAG CTAACCCATCATGCAGAACTATGC NB25 GTAAGTTGGGTATGCAACGCAATG CATTGCGTTGCATACCCAACTTAC NB26 CATACAGCGACTACGCATTCTCAT ATGAGAATGCGTAGTCGCTGTATG NB27 CGACGGTTAGATTCACCTCTTACA TGTAAGAGGTGAATCTAACCGTCG NB28 TGAAACCTAAGAAGGCACCGTATC GATACGGTGCCTTCTTAGGTTTCA NB29 CTAGACACCTTGGGTTGACAGACC GGTCTGTCAACCCAAGGTGTCTAG NB30 TCAGTGAGGATCTACTTCGACCCA TGGGTCGAAGTAGATCCTCACTGA NB31 TGCGTACAGCAATCAGTTACATTG CAATGTAACTGATTGCTGTACGCA NB32 CCAGTAGAAGTCCGACAACGTCAT ATGACGTTGTCGGACTTCTACTGG NB33 CAGACTTGGTACGGTTGGGTAACT AGTTACCCAACCGTACCAAGTCTG NB34 GGACGAAGAACTCAAGTCAAAGGC GCCTTTGACTTGAGTTCTTCGTCC NB35 CTACTTACGAAGCTGAGGGACTGC GCAGTCCCTCAGCTTCGTAAGTAG NB36 ATGTCCCAGTTAGAGGAGGAAACA TGTTTCCTCCTCTAACTGGGACAT NB37 GCTTGCGATTGATGCTTAGTATCA TGATACTAAGCATCAATCGCAAGC NB38 ACCACAGGAGGACGATACAGAGAA TTCTCTGTATCGTCCTCCTGTGGT NB39 CCACAGTGTCAACTAGAGCCTCTC GAGAGGCTCTAGTTGACACTGTGG NB40 TAGTTTGGATGACCAAGGATAGCC GGCTATCCTTGGTCATCCAAACTA NB41 GGAGTTCGTCCAGAGAAGTACACG CGTGTACTTCTCTGGACGAACTCC NB42 CTACGTGTAAGGCATACCTGCCAG CTGGCAGGTATGCCTTACACGTAG NB43 CTTTCGTTGTTGACTCGACGGTAG CTACCGTCGAGTCAACAACGAAAG NB44 AGTAGAAAGGGTTCCTTCCCACTC GAGTGGGAAGGAACCCTTTCTACT NB45 GATCCAACAGAGATGCCTTCAGTG CACTGAAGGCATCTCTGTTGGATC NB46 GCTGTGTTCCACTTCATTCTCCTG CAGGAGAATGAAGTGGAACACAGC NB47 GTGCAACTTTCCCACAGGTAGTTC GAACTACCTGTGGGAAAGTTGCAC NB48 CATCTGGAACGTGGTACACCTGTA TACAGGTGTACCACGTTCCAGATG NB49 ACTGGTGCAGCTTTGAACATCTAG CTAGATGTTCAAAGCTGCACCAGT NB50 ATGGACTTTGGTAACTTCCTGCGT ACGCAGGAAGTTACCAAAGTCCAT NB51 GTTGAATGAGCCTACTGGGTCCTC GAGGACCCAGTAGGCTCATTCAAC NB52 TGAGAGACAAGATTGTTCGTGGAC GTCCACGAACAATCTTGTCTCTCA NB53 AGATTCAGACCGTCTCATGCAAAG CTTTGCATGAGACGGTCTGAATCT NB54 CAAGAGCTTTGACTAAGGAGCATG CATGCTCCTTAGTCAAAGCTCTTG NB55 TGGAAGATGAGACCCTGATCTACG CGTAGATCAGGGTCTCATCTTCCA NB56 TCACTACTCAACAGGTGGCATGAA TTCATGCCACCTGTTGAGTAGTGA NB57 GCTAGGTCAATCTCCTTCGGAAGT ACTTCCGAAGGAGATTGACCTAGC NB58 CAGGTTACTCCTCCGTGAGTCTGA TCAGACTCACGGAGGAGTAACCTG NB59 TCAATCAAGAAGGGAAAGCAAGGT ACCTTGCTTTCCCTTCTTGATTGA NB60 CATGTTCAACCAAGGCTTCTATGG CCATAGAAGCCTTGGTTGAACATG NB61 AGAGGGTACTATGTGCCTCAGCAC GTGCTGAGGCACATAGTACCCTCT NB62 CACCCACACTTACTTCAGGACGTA TACGTCCTGAAGTAAGTGTGGGTG NB63 TTCTGAAGTTCCTGGGTCTTGAAC GTTCAAGACCCAGGAACTTCAGAA NB64 GACAGACACCGTTCATCGACTTTC GAAAGTCGATGAACGGTGTCTGTC NB65 TTCTCAGTCTTCCTCCAGACAAGG CCTTGTCTGGAGGAAGACTGAGAA NB66 CCGATCCTTGTGGCTTCTAACTTC GAAGTTAGAAGCCACAAGGATCGG NB67 GTTTGTCATACTCGTGTGCTCACC GGTGAGCACACGAGTATGACAAAC NB68 GAATCTAAGCAAACACGAAGGTGG CCACCTTCGTGTTTGCTTAGATTC NB69 TACAGTCCGAGCCTCATGTGATCT AGATCACATGAGGCTCGGACTGTA NB70 ACCGAGATCCTACGAATGGAGTGT ACACTCCATTCGTAGGATCTCGGT NB71 CCTGGGAGCATCAGGTAGTAACAG CTGTTACTACCTGATGCTCCCAGG NB72 TAGCTGACTGTCTTCCATACCGAC GTCGGTATGGAAGACAGTCAGCTA NB73 AAGAAACAGGATGACAGAACCCTC GAGGGTTCTGTCATCCTGTTTCTT NB74 TACAAGCATCCCAACACTTCCACT AGTGGAAGTGTTGGGATGCTTGTA NB75 GACCATTGTGATGAACCCTGTTGT ACAACAGGGTTCATCACAATGGTC NB76 ATGCTTGTTACATCAACCCTGGAC GTCCAGGGTTGATGTAACAAGCAT NB77 CGACCTGTTTCTCAGGGATACAAC GTTGTATCCCTGAGAAACAGGTCG NB78 AACAACCGAACCTTTGAATCAGAA TTCTGATTCAAAGGTTCGGTTGTT NB79 TCTCGGAGATAGTTCTCACTGCTG CAGCAGTGAGAACTATCTCCGAGA NB80 CGGATGAACATAGGATAGCGATTC GAATCGCTATCCTATGTTCATCCG NB81 CCTCATCTTGTGAAGTTGTTTCGG CCGAAACAACTTCACAAGATGAGG NB82 ACGGTATGTCGAGTTCCAGGACTA TAGTCCTGGAACTCGACATACCGT NB83 TGGCTTGATCTAGGTAAGGTCGAA TTCGACCTTACCTAGATCAAGCCA NB84 GTAGTGGACCTAGAACCTGTGCCA TGGCACAGGTTCTAGGTCCACTAC NB85 AACGGAGGAGTTAGTTGGATGATC GATCATCCAACTAACTCCTCCGTT NB86 AGGTGATCCCAACAAGCGTAAGTA TACTTACGCTTGTTGGGATCACCT NB87 TACATGCTCCTGTTGTTAGGGAGG CCTCCCTAACAACAGGAGCATGTA NB88 TCTTCTACTACCGATCCGAAGCAG CTGCTTCGGATCGGTAGTAGAAGA NB89 ACAGCATCAATGTTTGGCTAGTTG CAACTAGCCAAACATTGATGCTGT NB90 GATGTAGAGGGTACGGTTTGAGGC GCCTCAAACCGTACCCTCTACATC NB91 GGCTCCATAGGAACTCACGCTACT AGTAGCGTGAGTTCCTATGGAGCC NB92 TTGTGAGTGGAAAGATACAGGACC GGTCCTGTATCTTTCCACTCACAA NB93 AGTTTCCATCACTTCAGACTTGGG CCCAAGTCTGAAGTGATGGAAACT NB94 GATTGTCCTCAAACTGCCACCTAC GTAGGTGGCAGTTTGAGGACAATC NB95 CCTGTCTGGAAGAAGAATGGACTT AAGTCCATTCTTCTTCCAGACAGG NB96 CTGAACGGTCATAGAGTCCACCAT ATGGTGGACTCTATGACCGTTCAG -
Rapid barcode sequences
Below is a full list of the rapid barcode sequences used across the rapid-based barcoding kits, with the component acronym for the specific sequencing kit. The barcodes in our rapid-based kits are shipped at 10 µM.
Note: These are not the full sequences due to proprietory information.
In addition to the barcodes, there are also flanking sequences which add an extra level of context during analysis.
Rapid barcoding kits
Rapid Barcoding Kit 24 V14 (SQK-RBK114.24): RB01-24
Rapid Barcoding Kit 96 V14 (SQK-RBK114.96): RB01-96
Legacy: Rapid Barcoding Kit (SQK-RBK004): RB01-12
Rapid Barcoding Kit 96 (SQK-RBK110.96): RB01-96Barcode flanking sequence:
5' - GCTTGGGTGTTTAACC - barcode - GTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTGCGCCGCTTCA - 3'
PCR barcoding kits
PCR-cDNA Barcoding Kit 24 V14 (SQK-PCB114.24): BP01-24
Legacy PCR-cDNA Barcoding Kit 24 (SQK-PCB111.24): BP01-24
Legacy: PCR-cDNA Barcoding Kit (SQK-PCB109): BP01-12
Legacy: PCR Barcoding Kit (SQK-PBK004): BP01-12Barcode flanking sequence:
Top strand: 5' - ATCGCCTACCGTGA - barcode - TTGCCTGTCGCTCTATCTTC - 3'
Bottom strand: 5' - ATCGCCTACCGTGA - barcode - TCTGTTGGTGCTGATATTGC - 3'The top and bottom barcode flanking sequences are different to avoid 5' and 3' end sequences annealing to each other and forming a loop.
16S barcoding kits
16S Barcoding Kit 24 V14 (SQK-16S114.24): 16S01-24
Legacy 16S Barcoding Kit 1-24 (SQK-16S024): 16S01-24
Legacy: 16S Barcoding Kit (SQK-RAB204): 16S01-12Barcode flanking sequence:
Forward 16S primer: 5' - ATCGCCTACCGTGAC - barcode - AGAGTTTGATCMTGGCTCAG - 3'
Reverse 16S primer: 5' - ATCGCCTACCGTGAC - barcode - CGGTTACCTTGTTACGACTT - 3'The 3' flanking sequence of the forward primer contains a wobble base (denoted by M; in the primer the base is either an A or a C) in a variable region of the 16S gene.
Rapid PCR barcoding kits
Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24): RLB01-24
Legacy: Rapid PCR Barcoding Kit (SQK-RPB004): RLB01-12ABarcode flanking sequence:
5' - ATCGCCTACCGTGAC - barcode - CGTTTTTCGTGCGCCGCTTC - 3'
PCR barcoding expansions
PCR Barcoding Expansion 1-12 (EXP-PBC001): BC01-12
PCR Barcoding Expansion 1-96 (EXP-PBC096): BC01-96Barcode flanking sequence:
5' - GGTGCTG - barcode - TTAACCT - 3'Component Sequence BP01 / BC01 / RB01 / 16S01 / RLB01 AAGAAAGTTGTCGGTGTCTTTGTG BP02 / BC02 / RB02 / 16S02 / RLB02 TCGATTCCGTTTGTAGTCGTCTGT BP03 / BC03 / RB03 / 16S03 / RLB03 GAGTCTTGTGTCCCAGTTACCAGG BP04 / BC04 / RB04 / 16S04 / RLB04 TTCGGATTCTATCGTGTTTCCCTA BP05 / BC05 / RB05 / 16S05 / RLB05 CTTGTCCAGGGTTTGTGTAACCTT BP06 / BC06 / RB06 / 16S06 / RLB06 TTCTCGCAAAGGCAGAAAGTAGTC BP07 / BC07 / RB07 / 16S07 / RLB07 GTGTTACCGTGGGAATGAATCCTT BP08 / BC08 / RB08 / 16S08 / RLB08 TTCAGGGAACAAACCAAGTTACGT BP09 / BC09 / RB09 / 16S09 / RLB09 AACTAGGCACAGCGAGTCTTGGTT BP10 / BC10 / RB10 / 16S10 / RLB10 AAGCGTTGAAACCTTTGTCCTCTC BP11 / BC11 / RB11 / 16S11 / RLB11 GTTTCATCTATCGGAGGGAATGGA BP12 / BC12 / RB12 / 16S12 CAGGTAGAAAGAAGCAGAATCGGA RLB12 (in current chemistry kits V14+ / RLB012A in legacy kits) GTTGAGTTACAAAGCACCGATCAG BP13 / BC13 / RB13 / 16S13 / RLB13 AGAACGACTTCCATACTCGTGTGA BP14 / BC14 / RB14 / 16S14 / RLB14 AACGAGTCTCTTGGGACCCATAGA BP15 / BC15 / RB15 / 16S15 / RLB15 AGGTCTACCTCGCTAACACCACTG BP16 / BC16 / RB16 / 16S16 / RLB16 CGTCAACTGACAGTGGTTCGTACT BP17 / BC17 / RB17 / 16S17 / RLB17 ACCCTCCAGGAAAGTACCTCTGAT BP18 / BC18 / RB18 / 16S18 / RLB18 CCAAACCCAACAACCTAGATAGGC BP19 / BC19 / RB19 / 16S19 / RLB19 GTTCCTCGTGCAGTGTCAAGAGAT BP20 / BC20 / RB20 / 16S20 / RLB20 TTGCGTCCTGTTACGAGAACTCAT BP21 / BC21 / RB21 / 16S21 / RLB21 GAGCCTCTCATTGTCCGTTCTCTA BP22 / BC22 / RB22 / 16S22 / RLB22 ACCACTGCCATGTATCAAAGTACG BP23 / BC23 / RB23 / 16S23 / RLB23 CTTACTACCCAGTGAACCTCCTCG BP24 / BC24 / RB24 / 16S24 / RLB24 GCATAGTTCTGCATGATGGGTTAG BC25 / RB25 GTAAGTTGGGTATGCAACGCAATG BC26 / RB26 CATACAGCGACTACGCATTCTCAT BC27 / RB27 CGACGGTTAGATTCACCTCTTACA BC28 / RB28 TGAAACCTAAGAAGGCACCGTATC BC29 / RB29 CTAGACACCTTGGGTTGACAGACC BC30 / RB30 TCAGTGAGGATCTACTTCGACCCA BC31 / RB31 TGCGTACAGCAATCAGTTACATTG BC32 / RB32 CCAGTAGAAGTCCGACAACGTCAT BC33 / RB33 CAGACTTGGTACGGTTGGGTAACT BC34 / RB34 GGACGAAGAACTCAAGTCAAAGGC BC35 / RB35 CTACTTACGAAGCTGAGGGACTGC BC36 / RB36 ATGTCCCAGTTAGAGGAGGAAACA BC37 / RB37 GCTTGCGATTGATGCTTAGTATCA BC38 / RB38 ACCACAGGAGGACGATACAGAGAA BC39 / RB39 CCACAGTGTCAACTAGAGCCTCTC BC40 / RB40 TAGTTTGGATGACCAAGGATAGCC BC41 / RB41 GGAGTTCGTCCAGAGAAGTACACG BC42 / RB42 CTACGTGTAAGGCATACCTGCCAG BC43 / RB43 CTTTCGTTGTTGACTCGACGGTAG BC44 / RB44 AGTAGAAAGGGTTCCTTCCCACTC BC45 / RB45 GATCCAACAGAGATGCCTTCAGTG BC46 / RB46 GCTGTGTTCCACTTCATTCTCCTG BC47 / RB47 GTGCAACTTTCCCACAGGTAGTTC BC48 / RB48 CATCTGGAACGTGGTACACCTGTA BC49 / RB49 ACTGGTGCAGCTTTGAACATCTAG BC50 / RB50 ATGGACTTTGGTAACTTCCTGCGT BC51 / RB51 GTTGAATGAGCCTACTGGGTCCTC BC52 / RB52 TGAGAGACAAGATTGTTCGTGGAC BC53 / RB53 AGATTCAGACCGTCTCATGCAAAG BC54 / RB54 CAAGAGCTTTGACTAAGGAGCATG BC55 / RB55 TGGAAGATGAGACCCTGATCTACG BC56 / RB56 TCACTACTCAACAGGTGGCATGAA BC57 / RB57 GCTAGGTCAATCTCCTTCGGAAGT BC58 / RB58 CAGGTTACTCCTCCGTGAGTCTGA BC59 / RB59 TCAATCAAGAAGGGAAAGCAAGGT BC60 / RB60 CATGTTCAACCAAGGCTTCTATGG BC61 / RB61 AGAGGGTACTATGTGCCTCAGCAC BC62 / RB62 CACCCACACTTACTTCAGGACGTA BC63 / RB63 TTCTGAAGTTCCTGGGTCTTGAAC BC64 / RB64 GACAGACACCGTTCATCGACTTTC BC65 / RB65 TTCTCAGTCTTCCTCCAGACAAGG BC66 / RB66 CCGATCCTTGTGGCTTCTAACTTC BC67 / RB67 GTTTGTCATACTCGTGTGCTCACC BC68 / RB68 GAATCTAAGCAAACACGAAGGTGG BC69 / RB69 TACAGTCCGAGCCTCATGTGATCT BC70 / RB70 ACCGAGATCCTACGAATGGAGTGT BC71 / RB71 CCTGGGAGCATCAGGTAGTAACAG BC72 / RB72 TAGCTGACTGTCTTCCATACCGAC BC73 / RB73 AAGAAACAGGATGACAGAACCCTC BC74 / RB74 TACAAGCATCCCAACACTTCCACT BC75 / RB75 GACCATTGTGATGAACCCTGTTGT BC76 / RB76 ATGCTTGTTACATCAACCCTGGAC BC77 / RB77 CGACCTGTTTCTCAGGGATACAAC BC78 / RB78 AACAACCGAACCTTTGAATCAGAA BC79 / RB79 TCTCGGAGATAGTTCTCACTGCTG BC80 / RB80 CGGATGAACATAGGATAGCGATTC BC81 / RB81 CCTCATCTTGTGAAGTTGTTTCGG BC82 / RB82 ACGGTATGTCGAGTTCCAGGACTA BC83 / RB83 TGGCTTGATCTAGGTAAGGTCGAA BC84 / RB84 GTAGTGGACCTAGAACCTGTGCCA BC85 / RB85 AACGGAGGAGTTAGTTGGATGATC BC86 / RB86 AGGTGATCCCAACAAGCGTAAGTA BC87 / RB87 TACATGCTCCTGTTGTTAGGGAGG BC88 / RB88 TCTTCTACTACCGATCCGAAGCAG BC89 / RB89 ACAGCATCAATGTTTGGCTAGTTG BC90 / RB90 GATGTAGAGGGTACGGTTTGAGGC BC91 / RB91 GGCTCCATAGGAACTCACGCTACT BC92 / RB92 TTGTGAGTGGAAAGATACAGGACC BC93 / RB93 AGTTTCCATCACTTCAGACTTGGG BC94 / RB94 GATTGTCCTCAAACTGCCACCTAC BC95 / RB95 CCTGTCTGGAAGAAGAATGGACTT BC96 / RB96 CTGAACGGTCATAGAGTCCACCAT