-
Ligation-based sequencing kits
These are the previously available Ligation Sequencing Kits available using our Kit 9, 10 and 12 chemistry:
- Ligation Sequencing Kit (SQK-LSK112)
- Ligation Sequencing Kit XL (SQK-LSK112-XL)
- Ligation Sequencing Kit (SQK-LSK110)
- Ligation Sequencing Kit (SQK-LSK109)
- Ligation Sequencing Kit XL (SQK-LSK109-XL)
- Cas9 Sequencing Kit (SQK-CS9109)
- Native Barcoding Kit 1-12 (EXP-NBD104)
- Native Barcoding Kit 13-24 (EXP-NBD114)
- Native Barcoding Kit 96 (EXP-NBD196)
- Multiplex Ligation Sequencing Kit XL (SQK-MLK111.96-XL)
-
Ligation Sequencing Kits
SQK-LSK112
The Ligation Sequencing Kit (SQK-LSK112) was our previous upgrade from the Ligation Sequencing Kit (SQK-LSK110) and is optimised for high accuracy. The upgrade included a new sequencing adapter (Adapter Mix H, AMX H) which improved accuracies to over 99% (Q20+), required lower flow cell loading amounts and contained the fuel fix technology to run long experiments without the need for fuel addition during a run.
Note: This kit (SQK-LSK112) is a legacy product and will soon be discontinued. We recommend all customers to upgrade to the latest chemistry for their relevant kit which is available on the Store. If customers require further support for any ongoing critical experiments using a Legacy product, please contact Technical Support via email: support@nanoporetech.com.
SQK-LSK110
The Ligation Sequencing Kit (SQK-LSK110) is an upgrade from the Ligation Sequencing Kit (SQK-LSK109) and is optimised for high output. The upgrade contains new fuel fix adapter (Adapter Mix F, or AMX-F) to use significantly less fuel during a sequencing run to improve sequencing output. We have also upgraded the loading beads to Loading Beads II (LBII) and Loading Solution (LS) for users who do not use loading beads for their experiments.
SQK-LSK109
This is a ligation-based sequencing kit to offer a flexible method of preparing sequencing libraries from dsDNA (e.g. gDNA, cDNA or amplicons) and is optimised to generate maximum output. Since release, this kit has undergone multiple upgrades and we recommend customers to upgrade to our latest chemistry to achieve the best results.
These are the previously available expansion packs available to work alongside the Ligation Sequencing Kits to enable users to barcode their samples for more efficient use of the kits.
Barcoding expansion packs available for the Ligation Sequencing Kits:
- Compatible with Ligation Sequencing Kit (SQK-LSK109):
- Native Barcoding Expansion 1-12 (EXP-NBD104)
- Native Barcoding Expansion 13-24 (EXP-NBD114)
- Native Barcoding Expansion 96 (EXP-NBD196)
- Compatible with Ligation Sequencing Kit (SQK-LSK109):
-
Cas9 Sequencing Kit (SQK-CS0109)
The Cas9 Sequencing Kit (SQK-CS9109) uses ligation-based chemistry and is a fast and flexible way to target specific regions of interest within a genome without amplification, increasing the depth of coverage. This is particularly useful for areas not amenable to PCR, “dark” areas of the genome, or for users wishing to target specific regions but maintain DNA modifications. The Targeted, amplification-free DNA sequencing using CRISPR/Cas document explains how to design and order crRNA probes and shares best practices for performing targeted sequencing.
Using the recommended 5 µg high molecular weight gDNA, the DNA is dephosphorylated before adding Cas9 ribonucleoprotein particles (RNPs) with bound crRNA and tracrRNA to the gDNA for binding and cleavage of the Region of Interest (ROI). The cleavage by Cas9 reveals blunt ends with 5’ phosphates which are dA-tailed to prepare the blunt ends for barcode ligation. Sequencing adapters are then ligated to the Cas9-cut sides. Finally, a clean-up step is performed to remove excess adapters and the library is sequenced.