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Introduction to expansion packs
Expansion packs are available to be used alongside the sequencing kits and include extra reagents or barcodes for more efficient use of our sequencing kits.
Below is an overview of our current expansion kits, however, the store expansion pack page also includes additional simple expansions containing additional reagents.
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Sequencing Auxiliary Vial
This expansion pack (EXP-AUX003) is recommended for users who want to split a library across multiple flow cells, need to add more library to an existing run, or wish to top up a flow cell with an additional library. This kit contains sufficient reagents for six reactions with our V14 kits, including additional flow cell priming reagents.
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Flow Cell Wash Kit and XL
This kit (EXP-WSH004) is recommended for users who want to run multiple sequencing libraries on the same flow cell and is compatible with R9.4.1 and R10.4.1 flow cells. It can also be used for when a flow cell has accumulated a high number of ‘unavailable’ pores during a run and users want to revert these pores to the available state.
This kit provides a highly effective means of removing a library that has been loaded onto a flow cell. Once the flow cell is washed, it is available immediately for use again or to store. There are sufficient reagents for six flow cell washes. The Flow Cell Wash Kit XL (EXP-WSH004-XL) is a scaled up version of the kit, providing enough reagents for 48 reactions.
We recommend barcoding samples when running multiple samples sequentially to allow for filtering of sequences remaining from previous runs as there may be some residual DNA on the flow cell.
A sample with four barcodes was sequencing before the flow cell was washed using EXP-WSH004 before a samples with four different barcodes was loaded. This was repeated for a third sequencing run. We repeated this using EXP-WSH003 to illustrate the comparable washing between the wash kits. -
Flow Cell Priming Kit
We have released a separate expansion containing just V14 flow cell priming reagents. These are to be used alongside our sequencing kits to prime a MinION/GridION and PromethION Flow Cells, or to add more library to a flow cell during a sequencing experiment for another six reactions.
Note: The Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114) requires the specific flow cell priming reagents included in the kit and is not compabitible with the Flow Cell Priming Kit (EXP-FLP004).
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PCR Expansion
This expansion pack (EXP-PCA001) contains PCR Primers (PRM) and PCR Adapters (PCA) and is recommended for users needing reagents for a PCR-based sequencing experiment with the Ligation Sequencing Kit V14 (SQK-LSK114). This kit is recommended when users have a low starting input that requires PCR for preparing enough DNA for sequencing. For more information, please see the relevant protocol:
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Native Barcoding Auxiliary Kit V14
This expansion (EXP-NBA114) contains additional library preparation reagents for use with unused barcodes in the Native Barcoding Kits 24 and 96 V14 (SQK-NBD114.24 and SQK-NBD114.96). There are sufficient reagents for another 12 reactions supplied with this kit.
Note: This kit must by used in conjunction with the Sequencing Auxiliary Vials V14 expansion (EXP-AUX003) to provide the flow cell priming reagents.
Note: This Product Contains AMPure XP Reagent Manufactured by Beckman Coulter, Inc. and can be stored at -20°C with the kit without detriment to reagent stability.
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Rapid Adapter Auxiliary V14
This expansion (EXP-RAA114) provides additional Rapid Adapter (RA) and Adapter Buffer (ADB) to maximise any unused barcodes in the Rapid Barcoding Kit 24 and 96 V14 (SQK-RAD114.24 and SQK-RAD114.96).
Note: This kit must by used in conjunction with the Sequencing Auxiliary Vials V14 expansion (EXP-AUX003) to provide the flow cell priming reagents.