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Sequencing kit reagents
Below is a list of our common reagents included in our sequencing kits.
Ligation Adapter (LA)
The sequencing adapter is ligated onto the dA-tailed DNA fragments during library preparation. A motor protein is loaded onto the sequencing adapter and regulates the DNA passage through the nanopore.Native Adapter (NA)
The sequencing adapter ligates to the native barcodes ligated onto the dA-tailed DNA fragments during the library preparation of the native barcoding kits. A motor protein is loaded onto the sequencing adapter and regulates the DNA passage through the nanopore.Fragmentation Mix (FRA)
This reagent contains a transposase to fragment DNA strands during library preparation and simultaneously attaches transposase adapters to the DNA ends.Rapid Adapter (RA)
The sequencing adapter is attached to the transposase adapters in the Fragmentation Mix (FRA). A motor protein is loaded onto the sequencing adapter and regulates the DNA passage through the nanopore.Adapter Buffer (ADB)
This buffer is used in conjunction with the Rapid Adapter (RA) during the adapter attachment step to reach the correct concentration for optimal adaptation.Native Barcodes (NB01-96)
Barcodes are a known sequence used to identify samples in a pooled library. The Native Barcodes are specific to this kit and are ligated onto the DNA ends after end-prep. Native barcodes are shipped at 640 nM.EDTA (EDTA)
This reagent is used during the barcoding step of the native barcoding kits to stop the barcode ligation reaction after incubation.AMPure XP Beads (AXP)
These beads are manufactured by Beckman Coulter, Inc. and are used in the clean up steps of library preparations to purify a reaction e.g. excess barcodes or transposases present in a reaction.Sequencing Buffer (SB)
The sequencing buffer provides fuel and the optimal chemical conditions for powering DNA translocation through the nanopore. In SQK-LSK114, this component has been upgraded to improve sequencing by reducing variability in current levels during experiment runs.Short and Long Fragment Buffers
Short Fragment Buffer (SFB) is used to enrich fragments of all sizes after adapter ligation.
Long Fragment Buffer (LFB) is used to enrich fragments of ≥3 kb after adapter ligation.Ligation Buffer (LNB)
This buffer is used to aid ligation of the sequencing adapters.Elution Buffer (EB)
This buffer is optimised to keep the integrity of the motor protein on the Y-shaped adapter.Library Beads (LIB)
These beads help reduce frequency of blocking.Library Solution (LIS)
This reagent is the same solution that the Library Beads are stored in and are used to ease the loading of viscous libraries.Flow Cell Flush (FCF) and Flow Cell Tether (FCT)
These reagents are combined to make up the flow cell priming mix. The tether is used to bring the DNA strand to the membrane and to improve DNA capture by approximately 10,000-fold compared with capture without tether.DNA Control Sample (DCS)
The DNA Control Sample (DCS) is a 3.6 kb amplicon of the Lambda phage genome. The control sample is included in the library and added to DNA samples during DNA repair and end-prep for troubleshooting purposes.Lambda DNA (LMD)
The Lambda DNA (LMD) is a pure, high-quality DNA sample that can be used to prepare a library and run a control experiment. The lambda control protocols allows a new user to practise the process of library preparation, and can also be used to troubleshoot a poorly performing experiments.